Tetraspanin CD82 interaction with cholesterol promotes extracellular vesicle–mediated release of ezrin to inhibit tumour cell movement
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چکیده
منابع مشابه
Tetraspanin CD82 controls the association of cholesterol-dependent microdomains with the actin cytoskeleton in T lymphocytes: relevance to co-stimulation.
T-cell activation is initiated by the concerted engagement of the T-cell receptor and different co-stimulatory molecules, and requires cytoskeleton-dependent membrane dynamics. Here, we have studied the relationships between tetraspanins, cytoskeleton and raft microdomains, and their relevance in T-cell signaling. Localization studies and density-gradient flotation experiments indicate that par...
متن کاملCell Boundary Detection for Quantitative Studies of the Role of Tetraspanin CD82 in Cell-cell Adhesion
Cell-cell adhesion is mediated by interaction of a number of protein complexes residing on the cell plasma membranes. Disruption of these complexes affects structural and functional integrity of tissues. It has been hypothesized that a membrane protein tetraspanin CD82, a known metastasis suppressor, may play a role in regulating cell adhesion. Two proteins of interest (PG and Dsg2) have been f...
متن کاملTetraspanin CD82 Inhibits Protrusion and Retraction in Cell Movement by Attenuating the Plasma Membrane-Dependent Actin Organization
To determine how tetraspanin KAI1/CD82, a tumor metastasis suppressor, inhibits cell migration, we assessed which cellular events critical for motility are altered by KAI1/CD82 and how KAI1/CD82 regulates these events. We found that KAI1/CD82-expressing cells typically exhibited elongated cellular tails and diminished lamellipodia. Live imaging demonstrated that the polarized protrusion and ret...
متن کاملRegulation of urokinase receptor proteolytic function by the tetraspanin CD82.
The high affinity interaction between the urokinase-type plasminogen activator (uPA) and its glycolipid-anchored cellular receptor (uPAR) promotes plasminogen activation and the efficient generation of pericellular proteolytic activity. We demonstrate here that expression of the tetraspanin CD82/KAI1 (a tumor metastasis suppressor) leads to a profound effect on uPAR function. Pericellular plasm...
متن کاملTetraspanin CD82 regulates compartmentalisation and ligand-induced dimerization of EGFR.
We have previously shown that CD82, a transmembrane protein of the tetraspanin superfamily is associated with EGFR and has a negative effect on EGF-induced signalling (Odintsova, E., Sugiura, T. and Berditchevski, F. (2000) Curr. Biol. 10, 1009-1012). Here we demonstrate that CD82 specifically attenuates ligand-induced dimerization of EGFR. The recombinant soluble large extracellular loop of CD...
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ژورنال
عنوان ژورنال: Journal of Extracellular Vesicles
سال: 2019
ISSN: 2001-3078,2001-3078
DOI: 10.1080/20013078.2019.1692417